Coding
Part:BBa_K3096001:Design
Designed by: Lina Widerspick, Patrick Mueller, Famke Baeuerle, Jakob Keck Group: iGEM19_Tuebingen (2019-10-14)
The Cas3 Nuclease is part of the Type I CRISPR/Cas system in E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Two PstI digestion sites were mutated via wobble base exchange
Source
E.coli K12/ MG1655